is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 96- and 384-well microplates. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. 0 out of 5 $ 14,499. Artel MVS Bundle DM Std/800TSNB Reader Details. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. the Gemini XPS reader. Plate formats. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. QuickSync. the iD5 reader’s filter system. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. plates < 100 amol (384-well) 1 0200-7018. The plate was also read at z height 1 mm with varying delay and integration times for the donor and acceptor reads. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. More Product Information. Summary of Contents for Molecular Devices SpectraMax M2. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Supplier: Molecular Devices. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Temperature control. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The FLIPR 384 system comes complete with 384. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of. #PLUS 384) UV-transparent 96-well microplates (Corning cat. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. (96- and 384-well with pipetting) 6-384. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). X. for ELISAs and most cell-based assays. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. ". When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andCompany Molecular Devices, LLC; Price Get Quote; Catalog Number CSI / CSI FL; Type Cell Imaging System; Measuring Time White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min; Plate Formats 6, 12, 24, 96 & 384-well SBS microplates; Applications Cell Line Development, Fluorescence, Time-LapseNext-generation luminescence plate reader. ZERO BIAS - scores, article reviews, protocol conditions and more. 0. . High base plate design is compatible with automated handling (gripping) and bar coding. 340 PC384. Inquiry Basket. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. EMax. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. Comprehensive solution. SpectraMax M2 and M2e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. 100 fmol 1536-well (8 µL) 0. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . Validation made easy -The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader, spectramax i3x. Integrated stacker option. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The system provides automation for up to 50 microplates for easy walkaway automation. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. A control well was set up to verify. 384-WELL SBS STERILE MICROPLATE. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. Microplates, cuvettes (via adapter) Detectors. All measurements were performed in six replicates and cell toxicity was calculated as the percentage of the OD value of untreated or treated. 96 deep well plate: packed in sleeves of 6 , 24 per case. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. Microplate readers can be used to quantify this turbidity to monitor events such as cell growth or to perform solubility screens. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. Microseal 384-Well PCR Plates. Instrument pricing. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. x. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. VMax . Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. x. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. A snapshot of the Settings screen is shown in Figure 2. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Microplate format. Read one sample or up to 384. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. 50 μL total well volume, with recommended working volume of 5-40 μL. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. All stand-alone instrument functions can be controlled using the software. 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. For nearly 30 years, Molecular Devices has provided scientists with . The Standard Thermo Scientific 384-well plate, white, is compatible with the majority of 384 block PCR and sequencing instruments. Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. (or plate reader). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 039. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Description. Get Quote . Industry standard disposable (choose from recommended list). 2. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. e. TNF standard curve. 과 같은 Parameter를 검사하여 평가할 수 있습니다. To prevent evaporation, the microplate was covered with a qPCR seal. (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). The VICTOR Nivo. These robust readers have been placed in labs from Antarctica to the. readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. X. Using Molecular Devices microplate readers and SoftMax. No accessories are needed and SoftMax® Pro Software. The SpectraMax ABS Plus reader utilizes Molecular . Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. x. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Turn the reader on and secure the USB cable. Rating: 3. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Visible absorbance -. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 2. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. 96- and 384-well microplates. Two high-energy xenon flash lamps: Reading capabilities. Round well design with a clear flat bottom 2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Three low volume 24-well bottom slides. REF (SpectraMax Plus 384 only) A reading of buffer, water, or air taken in the cuvette that is used as I 0 to calculate Absorbance or % Transmittance. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 3% in white 96- and 384-well microplates Time. 384-WELL 10MM-HEIGHT HIGH SAMPLE RECOVERY MICROPLATES WI/WO LID. 303 unable to cal dark current. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. Backed by the. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. Figure 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. The opaque walls prevent stray light from being detected from neighbouring. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. 312 gain calibration failed. Fluorescence Microplate Reader Comparison Chart. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. All stand-alone instrument functions can be controlled using the software. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 96/384-well plate formats. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. In addition, SoftMax Pro SoftwareAfter incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. #6590-0118) Filter 665/10 (Molecular Devices cat. The MultiWash+ Microplate Washer is an automated washer that is configurable for both 96- and 384- well plates. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. #N207) LAL Reagent Water (Lonza cat. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. Compare specifications and request a quote from leading suppliers. It can be performed using any well in the plate with a relatively strong fluorescent signal (i. Following the flushing of the machine as described in Section 3. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. 304 signal level saturation. RUO. 1. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. #6590-0118) Filter 665/10 (Molecular Devices cat. easy to use and read multiple plates very quickly. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. 7. x. NanoQuant plate. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. 6% for the on-board optics (monochromators). Outer wells aligned for proper viewing with FLIPR. Category 2 and 3 microplate readers use gradient heating as well. x. The SpectraTest validation. 8 mm distance between well bottoms to plate bottom. 4 Equipment. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Browse for different fluorescent readers below and compare across different instrument suppliers. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. Plus 384. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. x. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. Compact design. The FilterMax F3 can read 96-well and 384-well plates. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Loading Buffer was also added to a set of cell-free wells for background controls. For more sample throughput, the system canPlate Background OD, which was used for subsequent tests, was measured as follows. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. , April 14, 2022 – Molecular Devices, LLC. They perform rapid throughput cell-based assays while. Outer wells aligned for proper viewing with FLIPR. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). Turn on Plate Reader and wait for it to complete its start-up routine. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Note: These settings are based on necessary cookies. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The drawing shows a standard microplate of 15 mm height covered with a lid. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. 9. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. 384 only) Selects either 96-well or 384-well plate for a stand-alone read. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andThe Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. 00. 635. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. Add the new plate. 11 10 100 1000 0 20 40 60 80 100 120 Calcium Mobilization in WT3M1 CHO Cells EC 50 = 13. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Round well design with a solid black flat bottom2. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. $402. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Values are averages from 5 clean plates, each filled with 200 µL water/well. x. Contact Us Phone: +1-800-635-5577 Web: Email: info@moldev. 5 ml microcentrifuge tubes 35 mm Petri dishes 12 mm × 50 mm test tubes (optional). formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. After the reaction was complete, 5 µL of each sample was transferred into wells of a 384-well plate and detected on the microplate readers using the instrument settings listed in Table 1. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit. 76 ± 0. ©2022. Dual injectors for kinetic assays. Unparalleled performance on a personalized platform. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels. in every plate. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Embla washers are durable and easy to maintain. Versa Max. C. 384 well plates 6–384 wells 6–384 wells 6–384 wells 6–1,536 wells (fluorometry, TRF, luminometry, AlphaScreen assays). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). . Can read 96 well plates (optional 384 well). Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. Suggested plates are black. The 384-well plate is limited to a maximum volume of a 30 µl addition in a much smaller diameter well, and using the 96. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. It comes standard with 4 wash/rinse bottles and 1 waste bottle for maximum washing flexibility. minutes and begin reading microplates with seven mouse clicks. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. 6-384 (96- and 384-well with pipetting) 96-well microplates. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Shaking: Linear, orbital, and double orbital. USA. Published Dec 16, 2020 | Updated Nov 07, 2022. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. 2. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Versa Max. Two different agonists, acetylcholine and carbachol, were tested. com. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. 2. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 96-384. For SpectraMax L or LMax II, LMax II 384 - no adapter used. 313 reference gain check fail. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Pro, Dual Read mode was selected. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. Washers configured for both 96- and 384-well plates. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. x. 1 OD at 230 nm. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . Synergy NEO multi-mode plate reader: BioTek: n/a: EL406 plate washer and bulk dispenser with wide-bore tip 10 μL cassette: BioTek: n/a:. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSupplier: Molecular Devices. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. 1. Next-generation luminescence plate reader. 1 x plates. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. tools to expand the boundaries of their research. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. x. In simplest terms, a plate reader measures and. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. A control well was set up to verify the accuracy of the standard curve. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3?Gurard-Levin describes his lab’s microplate reader selection: “In our labs, specific and refined optics are important, as well as the ability to use high density plates including those with 384- or 1536-wells. 20 attomol ATP per well. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. 2. Also refer to this Multimode Reader Comparison. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Total volume (except deep. 0200-6263. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Turn the. Ideal for miniaturized 384 well fluorescent. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Biotek Synergy 2 Plate Reader Multi-Mode. Figure 6. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. com Check our website for a current listing of worldwide distributors. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. 0. x.